Where did you get the toolkit apps from? Company written or purchased? If purchased, check with the vendor. If written internally, check with your IT department or the person who wrote them.
To test, remove the setting in your config.pro to NOT load the toolkit apps and see if Creo starts properly. Load 1 toolkit app at a time to see if there may be a conflict.
Ansys can be a bit tricky to get the folders set right.
I haven't done an Ansys plug-in since Ansys 2017, I think.
Unfortunately my notes are another system. I'll look on Monday and see if I can add anything.
Running the Ansys CAD configuration utility should set the config.pro to look for the right files.
Check the WBPlugInPE221.dat to be sure it is pointing to the Ansys install folders.
Also check the location of WBPlugInPE221.dat in your home folder config.pro.