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FDS13-Aquamarine
13-Aquamarine
July 19, 2016
Solved

Problem with 4 parameter logistic fit for immunoassay

  • July 19, 2016
  • 2 replies
  • 3603 views

I found an (old) pdf file on the web that uses a 4 parameter logistic fit for immunoassay. Unfortunately I must be overlooking something as it does not work in MC15. Are there more elegant ways to do this 4 parameter logistic fit?

Thanks in advance

Best answer by Werner_E

Here is an approach using "minerr"

2 replies

W
Werner_E25-Diamond I
25-Diamond I
July 19, 2016

Sure you can find a fit using a solve block with minerr and ever so often this has some advantages over using the built in "genfit" function.

But I doubt that the approach as posted by you ever worked in older Mathcad versions. We had to see the pdf to judge though.

Here is an approach using "genfit":

W
Werner_E25-Diamond IAnswer
25-Diamond I
July 19, 2016

Here is an approach using "minerr"

V
VladimirN24-Ruby III
24-Ruby III
July 20, 2016

Please find in the attachment another approximating model for your data.

F
FDS13-AquamarineAuthor
13-Aquamarine
July 23, 2016

Werner and Vladimir, thank you for your help. Fitting data to a standard in ELISA testing can be quite challenging so I appreciated all input.

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