Solved
OPC Aggregator reinitialization Connections by Service
At time i do reinitialization the runtime of the connections manually.
Is there a possibility, to trigger reinitialization the runtime of the connections, from the outside with a service?
I had completely overlooked the API function here.
The API function could be found via OPC Aggregator Administration and still had to be set up here.
Then release the necessary ports 57429 or 57529 on the server and network.
The documentation helped for the rest from page 175 or 218.
https://www.kepware.com/getattachment/e1943820-ef3c-4932-b055-4ef2a80ab863/kepserverex-manual.pdf
In thingworx dann noch ein Service erstellen mit der ContentLoaderFunctions:
Hope it helps others with the same problems.